|
KMID : 0894520060100040255
|
|
Development & Reproduction
2006 Volume.10 No. 4 p.255 ~ p.261
|
|
|
Effects of Endocrine Disruptors on the Expression of Estrogen Receptors in Ovary and Uterus from Immature Rats
|
|
Lee Kyeung-Yeup
Lee Sung-Ho
|
|
|
Abstract
|
|
|
|
Although some phytoes rogens might have beneficiary rather than adverse effects, most endocrine disrupting compounds(EDCs) are considered to be harmful to human and wildlife health through interfering the endocrine system. Previously we found that prepubertal exposure to genistein(GS), a well-known isoflavone phytoestrogen, could activate the reproductive system of immature female rats resulting precocious puberty. Interestingly, di(2-ethyl hexyl) phthalate(DEHP) exposure brought inverse result, a delayed puberty, in the same experimental regimen. In this study, we examined whether prepubertal exposure to GS or DEHP affect the gene expressions of estrogen receptors(ERα and ERβ) and LH receptor(LHR) which represent the maturational status of ovary and uterus in immature rats. GS (100 ㎎/㎏/day) was administered daily from postnatal day 25 to the day when the first vaginal opening(VO) was observed, and the animals were sacrificed on the next day(day 32). Similarly, DEHP(100 ㎎/㎏/day) was administered daily from postnatal day 25 through the day when the first V.O. in control group was observed, and the animals were sacrificed on the next day(day 36). To determine the transcriptional changes in the hormone receptors, total RNAs were extracted from ovary and uterus and were applied to semi-quantitative reverse transcription polymerase chain reaction(RT-PCR). In the GS group, the transcriptional activities of ERα, ERβ and LHR in uterus and LHR in ovary were significantly increased when compared to those of control group. In the DEHP group, the transcriptional activities of all the hormone receptors measured were significantly lowered when compared to those of control group. These alteration of the reproductive hormone receptor expressions in ovary and uterus might be represent the phenotypic aspects(secondary sexual characteristics) such as tissue weights and reproductive hormone levels during perinatal period in immature female rats.
|
|
|
KEYWORD
|
|
|
Genistein(GS), Di(2-ethyl hexyl)phthalate(DEHP), ERα, ERβ, LH receptor, Puberty
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|